Microbiology Lab 5
Acid Fast Stain, Bacterial Capsules & Bacterial Endospores

Acid Fast Stain



acid fast stain
The small pink bacilli above are Mycobacterium smegmatis, an acid fast bacteria because they retain the primary dye.  The darker staining cocci are Staphylococcus epidermidis , a non-acid fast bacterium.

 
 

Bacterial capsules



capsule stain
The capsule of Klebsiella pneumoniae  is demonstrated here.  This is a combination of a negative stain and a simple stain.  The pink bacilli can be seen within a clear capsule.

 
 
 
 

endospores
This is a demonstration of endospore formation of Bacillus anthracias.  The solid pink cells are vegetative bacteria.  The ones that appear green are endospores.

 
 
This is alternative spore stain.  The dye that stained the spores has faded in this old slide.  The solid red bacilli are vegetative cells 
The "bubble-like" cells contain endospores.