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| A student's depiction of a starch agar plate following the application of iodione. | A starch agar plate photographed following the addition of iodine. The colony on the right with the halo is positive |
Amylase, DNAase, Carbohydrate Fermentation, Triple Sugar Iron Agar, Urea hydrolysis
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| A student's depiction of a starch agar plate following the application of iodione. | A starch agar plate photographed following the addition of iodine. The colony on the right with the halo is positive |
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| A students depiction of DNAase results after the addition of 1 N HCl | A photograph of a DNAase plate following the addition of 1N HCl. The colony on the right with the clear halo is positive. |
Fermentation Testing Using Phenol Red Broth Base with Durham Tubes
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| No reaction No reaction Acid Acid + gas | No reaction No reaction Acid Acid + gas |
The end products of bacterial fermentation, regardless of the pathway, are acidic causing the pH indicator phenol red to turn yellow.
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Slant butt reactions of TSI from the top row left to right: A/A A/A w gas A/A w H2S Alk/A |
| A student's drawing of TSI with labeled results | Second row left to right;
Alk/A + gas Alk/A + H2S Alk/NR NR/NR |
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| A student drawing of a negative and positive urease test. | The yellow tube on the left is usease negative, while the pink colored tube on the right is urease positive |
The urease test still utilizes the ph indicator, phenol red. Note that positive reactions are red not yellow. The hydrolysis of urea ends in the prodcution of alkaline end products.